The move comes after he was demoted from campaign manager earlier in the week.
Paul Manafort has resigned as chairman of the Donald Trump's presidential campaign, effective Friday morning.
Read: See Awkward Moment CNN Anchor Tells Trump Adviser He's Down in the Polls: 'Says Who?'
In a statement, Trump said: “This morning Paul Manafort offered, and I accepted, his resignation from the campaign. I am very appreciative for his great work in helping to get us where we are today, and in particular his work guiding us through the delegate and convention process. Paul is a true professional and I wish him the greatest success."
The move comes less than three months before Election Day and after a week of shakeups within the GOP presidential nominee’s campaign.
Stephen Bannon and Kellyanne Conway were added to the campaign hierarchy as Manafort, the former campaign manager, was demoted to chairperson.
A source within the campaign told CNN that “Manafort told Trump he was becoming a distraction and he wanted to end that.”
The move comes less than a day after Trump expressed “regret” for some of the conduct he has exhibited on the campaign trail.
Speaking at a rally in Charlotte, North Carolina Thursday afternoon, Trump, who spoke with the use of Teleprompters, said: “Sometimes, in the heat of debate and speaking on a multitude of issues, you don’t choose the right words or you say the wrong thing. I have done that.
Read: Meeting of the Minds: Ivanka Trump Sits In on Her Father's National Security Summit
“Believe it or not, I regret it. And I do regret it, particularly where it may have caused personal pain. Too much is at stake for us to be consumed with these issues. But one thing I can promise you is this: I will always tell you the truth.”
He then wasted no time in getting a chance to take a dig at presidential rival, Hillary Clinton, saying: “So while sometimes I can be too honest, Hillary Clinton is the exact opposite: She never tells the truth.”
Watch: Less Than 3 Months Before The Election, Trump Shakes Up Top Campaign Staff Again