The letter threatened Trump and his family if he does not pull out of the race.
Donald Trump's son Eric received a suspicious white powder in an envelope on Thursday night, in what comes as the second threat towards the family in a week.
The letter was opened by Eric’s wife, Lara, a producer at INSIDE EDITION.
Read: GOP Debate Canceled After Trump Says He Won't Be There
The letter was addressed to Mr. And Mrs. Eric Trump and was delivered to their apartment building in Manhattan. Last night, when Lara got home from work, she opened it and a puff of white powder came out. She dropped the envelope immediately, washed her hands and left the apartment. The envelope had a Massachusetts postmark.
FBI and Secret Service agents converged on the apartment. The NYPD sent in a hazmat team. According to preliminary results, the powder does not appear to be toxic.
The letter warned the Republican frontrunner to drop out of the race or his family would be harmed.
It's the second major threat to Donald Trump this week. The hacker group Anonymous has declared “total war” on the candidate.
“Now is the time to fight fascism. We have a gift for you -- Trump's Social Security number, his cell phone number and other information that could help you investigate this fascist,” they said in a statement.
The Trump campaign says the FBI is taking the threat by Anonymous seriously.
"The government and law enforcement authorities are seeking the arrest of the people responsible for attempting to illegally hack Mr. Trump's accounts and telephone information," the statement from the FBI said.
Read: Trump's Kids Reveal What They Think of His Hair
The red hot campaign spilled into the streets of Brooklyn.
Video of two city sanitation workers were suspended after getting into it with a driver who complained about their truck blocking the street.
"Behave or you'll be deported," yelled one worker. "Where’s Trump? Where's Trump when you need him?"
Watch: Trump's New Ad Shows Hillary Clinton Barking Like a Dog