The drug test comes as Angelina Jolie holds a three-hour meeting with her divorce lawyer.
Brad Pitt has undergone drug testing as part of the investigation into child abuse allegations.
Read: Ex-Girlfriend of Brad Pitt - Not Jennifer Aniston - Speaks Out: 'He's Not This Crazy Monster'
The drug tests are considered "standard" for parents being investigated for allegedly abusing their kids and Pitt is "cooperating fully" with investigators. Pitt denies ever abusing his children.
Pitt was allegedly "wasted" when he had a physical altercation with 15-year-old son Maddox aboard a private jet two weeks ago.
Pitt and Jolie are trying to reach an amicable, behind-closed-doors agreement to avoid an ugly court battle over custody of their six children. People magazine reports they are deep in private negotiations.
Jolie met with her divorce lawyer, who was peppered with questions as he left a 3-hour meeting Wednesday.
Read: Was Johnny Depp Behind Brangelina Split? Biographer Says Jolie Has Crush on 'Pirates' Star
Us magazine reports that the pair argued constantly about how to raise the six kids.
"He became a big yeller," claims a source. "She would not tolerate him yelling at the kids. There were a few times Angie said she was terrified."
Watch: Jon Voight Speaks Out on Daughter Angelina Jolie's Divorce From Brad Pitt
